Unrestricted somatic stem cells (USSC) from human umbilical cord blood display uncommitted epigenetic signatures of the major stem cell pluripotency genes
نویسندگان
چکیده
منابع مشابه
Unrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres
BACKGROUND Human umbilical cord blood-derived unrestricted somatic stem cells (USSCs), which are capable of multilineage differentiation, are currently under investigation for a number of therapeutic applications. A major obstacle to their clinical use is the fact that in vitro expansion is still dependent upon fetal calf serum, which could be a source of pathogens. In this study, we investigat...
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Purpose: To isolate and purify unrestricted somatic stem cells from human umbilical cord blood and evaluation of their differentiation into chondrocyte in vitro.Materials and Methods: In this study cells from human umbilical cord blood were isolated and plated in flask. Colonies were performed after one week. To determine the kind of cells, 100000 cells were analysed with flowcytometry. Twenty ...
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Background The aim of this study was the ex vivo expansion of Umbilical Cord Blood hematopoietic stem cells on biocompatible nanofiber scaffolds. Materials and Methods CD133+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system by means of monocolonal antibody CD133 (microbeads) subsequently, flowcytometry method was done to asses...
متن کاملThe Effects of Glycogen Syntase Kinase-3 β Inhibitor on The Ostogenic Differentiation of Unrestricted Somatic Stem Cells (Ussc)
Purpose: Bone defects caused by injuries, trauma, cancer, surgical operation or joint dislocation have great impacts on general health. Considering that Wnt signalling is one of the important regulators of the stem cell control, we sought to examine if Wnt activation affects the osteogenic differentiation of the USSCs.Materials and Methods: In this study, we have treated USSCs cultured in osteo...
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Objective(s): In some previous studies, the extract of embryonic carcinoma cells (ECCs) and embryonic stem cells (ESCs) have been used to reprogram somatic cells to more dedifferentiated state. The aim of this study was to investigate the effect of mouse ESCs extract on the expression of some pluripotency markers in human adipose tissue-derived stem cells (ADSCs). Materials and Methods: Human A...
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ژورنال
عنوان ژورنال: Stem Cell Research
سال: 2011
ISSN: 1873-5061
DOI: 10.1016/j.scr.2010.08.003